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Image Repository: View Experiment
 

Experimental Details for YRC PIR ID: 10

Total Images: 4
Data Source: YRC Data Notes: Data file: EMY190s102902EM79.r3d
Z-sections: 1 Z plane depth: 0.20000 µm
Time series: 1 Panels: 1
Objective: 100X Binning: 2x2
Image size: 512x512 Pixel Size: 0.12758 µm x 0.12758 µm
Species:
Saccharomyces cerevisiae
 Strain: [+] EMY190
Visible Items:
SPC110 (YFP)
SPC42 (CFP)
Channels:
ex:500/em:545 (YELLOW)
ex:440/em:545 (FRET)
ex:440/em:480 (CYAN)
ex:0/em:500 (DIC)
FRET Donor: SPC42 FRET Acceptor: SPC110

Viewing Panel: 1 of 1

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Filter Channel: YELLOW
Visible Items:
SPC110 (YFP)
Excitation λ: 500 nm
Emission λ: 545 nm
Time point: 0.00s
X-coord: 2485.060000000
Y-coord: 1231.000000000
Z-coord: 0E-9
Photosensor: 690133
Exposure: 0.40 s
NDFilter: 0.00



Filter Channel: FRET Channel
Donor:
SPC42 (CFP)
Acceptor:
SPC110 (YFP)
Excitation λ: 440 nm
Emission λ: 545 nm
Time point: 1.00s
X-coord: 2485.060000000
Y-coord: 1231.000000000
Z-coord: 0E-9
Photosensor: 1753845
Exposure: 0.40 s
NDFilter: 0.00



Filter Channel: CYAN
Visible Items:
SPC42 (CFP)
Excitation λ: 440 nm
Emission λ: 480 nm
Time point: 3.00s
X-coord: 2485.060000000
Y-coord: 1231.000000000
Z-coord: 0E-9
Photosensor: 1756274
Exposure: 0.40 s
NDFilter: 0.00



Filter Channel: DIC
Time point: 4.00s
X-coord: 2485.060000000
Y-coord: 1231.000000000
Z-coord: 0E-9
Photosensor: 44
Exposure: 0.05 s
NDFilter: 0.00



Filter Channel: Merged
X-coord: 2485.060000000
Y-coord: 1231.000000000
Z-coord: 0E-9
Red Channel: CYAN
Green Channel: YELLOW
Blue Channel: DIC
UW
The YRC Public Image Repository is supported by grants P41GM103533 and GM40506 from National Institute of General Medical Sciences at the US National Institutes of Health.