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Genetic regulation of Dna2 localization during the DNA damage response
DNA damage signalling pathways are crucial for protecting genome stability in
all eukaryotes. Saccharomyces cerevisiae Dna2 has both helicase and nuclease activities
that are essential for Okazaki fragment maturation, and Dna2 is involved in long-range
DNA end resection at double-strand breaks. Dna2 forms nuclear foci in response to
DNA replication stress and to double-strand breaks. We find that Dna2-GFP focus
formation occurs mainly during S phase in unperturbed cells. Dna2 co-localizes in
nuclear foci with 25 DNA repair proteins that define recombination repair centers, in
response to phleomycin induced DNA damage. To systematically identify genes that
affect Dna2 focus formation, we crossed Dna2-GFP into 4293 non-essential gene deletion
mutants, and assessed Dna2-GFP nuclear focus formation after phleomycin treatment.
We identified 37 gene deletions that affect Dna2-GFP focus formation, 12 with fewer
foci and 25 with increased foci. Together these data comprise a useful resource for
understanding Dna2 regulation in response to DNA damage.
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The YRC Public Image Repository is supported by grants P41GM103533 and GM40506 from
National Institute of General Medical Sciences
at the US National Institutes of Health.
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